Roselle belongs to the Malvaceae family, it is an important source of vitamins, minerals, and the pharmacological effects of Roselle are related to its components such as flavonoids, anthocyanins, organic acids, phytosterols, polyphenols, and polysaccharides, some of which have antioxidant activities. In the present study, the storage life of fresh Roselle calyces was evaluated in terms of changes in terms of biochemical properties such as total phenol, flavonoid, anthocyanin contents and antioxidant activity for 5 to 30 days at 0, 4, 10 and ambient temperature (23) degrees Celsius. The results showed that the lower temperature (0 and 4 °C) preserves the appearance of the calyces during storage in the refrigerator. The shelf life based on the appearance of the product was higher at 0 and 4 °C than at 10 and 23 °C. In addition, fresh calyces exposed to 4°C accumulated higher amounts of phenolics, flavonoids, anthocyanins, and antioxidant activity than those exposed to 0°C to overcome severe cold stress. Consequently, for short-term storage or immediate consumption, it is desirable to store fresh calyces at 4°C to obtain high antioxidant content. However, if the goal is to extend the cold storage period, storage at 0°C is recommended.