Research in Plant Metabolites

Research in Plant Metabolites

Effect of salt stress on secondary metabolites and growth behavior of artichoke in vitro

Document Type : Original Article

Authors
Masoomeh RahimBarshi 1
Azim GhasemNejad 2
Mehdi Alizadeh 2
Ahad Yamechi 2
1 Master's degree in horticultural sciences - medicinal plants Gorgan University of Agricultural Sciences and Natural Resources
2 Associate Professor of Gorgan University of Agricultural Sciences and Natural Resources, Faculty of Plant Production
10.22034/jrpsm.2024.460256.1039
Abstract
Artichoke (Cynara scolymus) is a medicinal plant with nutritional and economic value that faces a large area of low water and saline lands. The present research was conducted in two separate experiments in the form of a completely randomized design with the aim of investigating the effect of salinity on the secondary metabolites of the mentioned plant. This experiment was carried out in two ways: explant culture in MS solid culture medium with concentrations (0, 50, 150, 300, 600, 1200 μM NaCl) and callus culture in the same medium with concentrations (0, 50, 150, 300, 600, 1200, 2000, 4000, 6000 and 75000 µM NaCl) were done. After four weeks, its physiological and biochemical characteristics were investigated. The results showed a decreasing trend in internal pigments with increasing salinity concentration. It was also observed that in explant culture, the fresh weight of callus decreased with increasing salinity (the highest fresh weight was related to the control and the lowest was related to the salinity level of 1200 micromolar) and in the callus culture, the highest fresh weight was related to the salinity level of 75000 micromolar. At all salinity levels, the density of pigments in explant culture samples was higher than in callus culture samples. Examining the changes of phenolic and antioxidant compounds showed that unlike explant culture in callus culture, there is a significant difference in the percentage of free radical inhibition, chlorogenic acid and caffeic acid
Keywords
Callus
Antioxidant
Chlorogenic Acid
Caffeic Acid
Environmental Conditions
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