Research in Plant Metabolites

Research in Plant Metabolites

Study of Pectin Extraction Methods: From Traditional Approaches to Advanced Technologies

Document Type : Original Article

Authors
Department of Bioengineering, School of Life Sciences Engineering, College of Interdisciplinary Science and Technologies, University of Tehran, Tehran, Iran
Abstract
Pectin is a widely used plant polysaccharide predominantly found in the plant cell wall, exhibiting varying structural and functional properties depending on its source and extraction method. This article provides a comprehensive review of different approaches for extracting pectin from commercial and non-commercial sources, with a focus on the importance of the source type and extraction method. Initially, conventional extraction methods such as acidic, alkaline, and aqueous extraction are examined. Although these methods are simple and common, they face limitations due to high energy consumption, use of corrosive materials, and the potential degradation of pectin structure. Subsequently, novel methods including microwave-assisted extraction, ultrasound, enzymes, pulsed electric fields, ohmic heating, high pressure, plasma, and subcritical water extraction are reviewed. Each of these techniques has been developed with the aim of improving yield, reducing processing time, preserving pectin structure, and minimizing chemical usage, while requiring specialized equipment and precise optimization conditions. This article, through an analysis of the advantages and disadvantages of each method, demonstrates that the selection of the extraction technique depends on factors such as the type of plant source, intended application, sensitivity of the extracted composition, cost, environmental considerations, and available infrastructure. This article can serve as a suitable guide for choosing the optimal pectin extraction method in various industrial and research applications.
Keywords

Abou-Elseoud, W. S., Hassan, E. A., & Hassan, M. L. (2021). Extraction of pectin from sugar beet pulp by enzymatic and ultrasound-assisted treatments. Carbohydrate Polymer Technologies and Applications, 2: 100042.
Abou-Shady, A., & El-Araby, H. (2025). A comprehensive analysis of the advantages and disadvantages of pulsed electric fields during soil electrokinetic remediation. International Journal of Environmental Science and Technology, 22(5): 3895-3925.
Asgari, K., Labbafi, M., Khodaiyan, F., Kazemi, M., & Hosseini, S. S. (2020). High-methylated pectin from walnut processing wastes as a potential resource: Ultrasound assisted extraction and physicochemical, structural and functional analysis. International Journal of Biological Macromolecules, 152: 1274-1282.
Basak, S., & Annapure, U. S. (2022). The potential of subcritical water as a “green” method for the extraction and modification of pectin: A critical review. Food Research International, 161: 111849.
Calce, E., Bugatti, V., Vittoria, V., & Luca, S. D. (2012). Solvent-free synthesis of modified pectin compounds promoted by microwave irradiation. Molecules, 17(10): 12234-12242.
Chaharbaghi, E., Khodaiyan, F., & Hosseini, S. S. (2017). Optimization of pectin extraction from pistachio green hull as a new source. Carbohydrate polymers, 173: 107-113.
Chen, J., Zhang, C., Xia, Q., Liu, D., Tan, X., Li, Y., & Cao, Y. (2020). Treatment with subcritical water-hydrolyzed citrus pectin ameliorated cyclophosphamide-induced immunosuppression and modulated gut microbiota composition in ICR mice. Molecules, 25(6): 1302.
Cho, E. H., Jung, H. T., Lee, B. H., Kim, H. S., Rhee, J. K., & Yoo, S. H. (2019). Green process development for apple-peel pectin production by organic acid extraction. Carbohydrate polymers, 204: 97-103.
Forouhar, A., Hamdami, N., Djelveh, G., Lecerf, D., Rihouey, C., Gardarin, C., ... & Michaud, P. (2024). Effects of high voltage dielectric barrier discharge on the extraction and properties of pectins from watermelon rinds. Innovative Food Science & Emerging Technologies, 91: 103558.
Gavahian, M., & Chu, R. (2022). Ohmic heating extraction at different times, temperatures, voltages, and frequencies: a new energy-saving technique for pineapple core valorization. Foods, 11(14): 2015.
Gavahian, M., Chu, Y. H., & Sastry, S. (2018). Extraction from food and natural products by moderate electric field: Mechanisms, benefits, and potential industrial applications. Comprehensive Reviews in Food Science and Food Safety, 17(4): 1040-1052.
Guandalini, B. B. V., Rodrigues, N. P., & Marczak, L. D. F. (2019). Sequential extraction of phenolics and pectin from mango peel assisted by ultrasound. Food Research International, 119: 455-461.
Haque, S. M., Kabir, A., Ratemi, E., Elzagheid, M., Appu, S. P., Ghani, S. S., & Sarief, A. (2025). Greener pectin extraction techniques: Applications and challenges. Separations, 12(3): 65.
Hosseini, S. S., Khodaiyan, F., & Yarmand, M. S. (2016a). Aqueous extraction of pectin from sour orange peel and its preliminary physicochemical properties. International journal of biological macromolecules, 82: 920-926.
Hosseini, S. S., Khodaiyan, F., & Yarmand, M. S. (2016b). Optimization of microwave assisted extraction of pectin from sour orange peel and its physicochemical properties. Carbohydrate polymers, 140: 59-65.
Hosseini, S. S., Khodaiyan, F., Kazemi, M., & Najari, Z. (2019). Optimization and characterization of pectin extracted from sour orange peel by ultrasound assisted method. International journal of biological macromolecules, 125: 621-629.
Hu, W., Zhao, Y., Yang, Y., Zhang, H., Ding, C., Hu, C., ... & Yuan, M. (2019). Microwave-assisted extraction, physicochemical characterization and bioactivity of polysaccharides from Camptotheca acuminata fruits. International Journal of Biological Macromolecules, 133: 127-136.
Huang, H. W., Wu, S. J., Lu, J. K., Shyu, Y. T., & Wang, C. Y. (2017). Current status and future trends of high-pressure processing in food industry. Food control, 72: 1-8.
Kazemi, M., Amiri Samani, S., Ezzati, S., Khodaiyan, F., Hosseini, S. S., & Jafari, M. (2021). High‐quality pectin from cantaloupe waste: Eco‐friendly extraction process, optimization, characterization and bioactivity measurements. Journal of the Science of Food and Agriculture, 101(15): 6552-6562.
Kazemi, M., Khodaiyan, F., & Hosseini, S. S. (2019b). Eggplant peel as a high potential source of high methylated pectin: Ultrasonic extraction optimization and characterization. LWT, 105: 182-189.
Kazemi, M., Khodaiyan, F., Hosseini, S. S., & Najari, Z. (2019a). An integrated valorization of industrial waste of eggplant: Simultaneous recovery of pectin, phenolics and sequential production of pullulan. Waste Management, 100: 101-111.
Luo, W. B., Han, Z., Zeng, X. A., Yu, S. J., & Kennedy, J. F. (2010). Study on the degradation of chitosan by pulsed electric fields treatment. Innovative Food Science & Emerging Technologies, 11(4): 587-591.
Ma, S., & Wang, Z. H. (2013). Pulsed electric field-assisted modification of pectin from sugar beet pulp. Carbohydrate Polymers, 92(2): 1700-1704.
Mao, G., Wu, D., Wei, C., Tao, W., Ye, X., Linhardt, R. J., ... & Chen, S. (2019). Reconsidering conventional and innovative methods for pectin extraction from fruit and vegetable waste: Targeting rhamnogalacturonan I. Trends in Food Science & Technology, 94: 65-78.
Marić, M., Grassino, A. N., Zhu, Z., Barba, F. J., Brnčić, M., & Brnčić, S. R. (2018). An overview of the traditional and innovative approaches for pectin extraction from plant food wastes and by-products: Ultrasound-, microwaves-, and enzyme-assisted extraction. Trends in food science & technology, 76: 28-37.
Mehta, N., S, J., Kumar, P., Verma, A. K., Umaraw, P., Khatkar, S. K., ... & Sazili, A. Q. (2022). Ultrasound-assisted extraction and the encapsulation of bioactive components for food applications. Foods, 11(19): 2973.
Misra, N. N., Keener, K. M., Bourke, P., Mosnier, J. P., & Cullen, P. J. (2014). In-package atmospheric pressure cold plasma treatment of cherry tomatoes. Journal of bioscience and bioengineering, 118(2): 177-182.
Najari, Z., Khodaiyan, F., Yarmand, M. S., & Hosseini, S. S. (2022). Almond hulls waste valorization towards sustainable agricultural development: Production of pectin, phenolics, pullulan, and single cell protein. Waste Management, 141: 208-219.
Nova, M. V., Nothnagel, L., Thurn, M., Travassos, P. B., Herculano, L. S., Bittencourt, P. R., ... & Bruschi, M. L. (2019). Development study of pectin/Surelease® solid microparticles for the delivery of L-alanyl-L-glutamine dipeptide. Food Hydrocolloids, 89: 921-932.
Pasandide, B., Khodaiyan, F., Mousavi, Z. E., & Hosseini, S. S. (2017). Optimization of aqueous pectin extraction from Citrus medica peel. Carbohydrate polymers, 178: 27-33.
Pasandide, B., Khodaiyan, F., Mousavi, Z., & Hosseini, S. S. (2018). Pectin extraction from citron peel: optimization by Box–Behnken response surface design. Food science and biotechnology, 27(4): 997-1005.
Pico, Y. (2013). Ultrasound-assisted extraction for food and environmental samples. TrAC Trends in Analytical Chemistry, 43: 84-99.
Pinkowska, H., Wolak, P., Krzywonos, M., & Złocińska, A. (2021). Comprehensive study of hydrothermal extraction of pectin from sugar beet pulp. Waste and Biomass Valorization, 12(8): 4587-4598.
Pourramezan, H., Khodaiyan, F., & Hosseini, S. S. (2022). Extraction optimization and characterization of pectin from sesame (Sesamum indicum L.) capsule as a new neglected by‐product. Journal of the Science of Food and Agriculture, 102(14): 6470-6480.
Putra, N. R., Aziz, A. H. A., Faizal, A. N. M., & Che Yunus, M. A. (2022). Methods and potential in valorization of banana peels waste by various extraction processes: In review. Sustainability, 14(17): 10571.
Raji, Z., Khodaiyan, F., Rezaei, K., Kiani, H., & Hosseini, S. S. (2017). Extraction optimization and physicochemical properties of pectin from melon peel. International journal of biological macromolecules, 98: 709-716.
Ripoll, C. S. S., & Hincapié-Llanos, G. A. (2023). Evaluation of sources and methods of pectin extraction from fruit and Vegetable wastes: A Systematic Literature Review (SLR). Food Bioscience, 51: 102278.
Rodsamran, P., & Sothornvit, R. (2019). Microwave heating extraction of pectin from lime peel: Characterization and properties compared with the conventional heating method. Food chemistry, 278: 364-372.
Sabater, C., Blanco-Doval, A., Montilla, A., & Corzo, N. (2021). Optimisation of an enzymatic method to obtain modified artichoke pectin and pectic oligosaccharides using artificial neural network tools. In silico and in vitro assessment of the antioxidant activity. Food Hydrocolloids, 110: 106161.
Salimi, A., Khodaiyan, F., Askari, G., & Hosseini, S. S. (2024). A zero-waste approach towards a sustainable waste management of apple: Extraction of value-added products and their application as edible coating. Food Hydrocolloids, 147: 109304.
Sarkis, J. R., Jaeschke, D. P., Tessaro, I. C., & Marczak, L. D. (2013). Effects of ohmic and conventional heating on anthocyanin degradation during the processing of blueberry pulp. LWT-Food Science and Technology, 51(1): 79-85.
Sharifi, A., Hamidi-Esfahani, Z., Gavlighi, H. A., & Saberian, H. (2022). Assisted ohmic heating extraction of pectin from pomegranate peel. Chemical Engineering and Processing-Process Intensification, 172: 108760.
Sivakumar, M., & Pandit, A. B. (2001). Ultrasound enhanced degradation of Rhodamine B: optimization with power density. Ultrasonics Sonochemistry, 8(3): 233-240.
Talha, M., Khalid, S., Maan, A. A., Tanveer, N., Khan, M. K. I., Asif, M., ... & Sarwar, A. (2024). Ohmic assisted extraction: a sustainable and environment friendly approach to substitute conventional extraction methods. Food Reviews International, 40(10): 3508-3529.
Wang, W., Ma, X., Xu, Y., Cao, Y., Jiang, Z., Ding, T., ... & Liu, D. (2015). Ultrasound-assisted heating extraction of pectin from grapefruit peel: Optimization and comparison with the conventional method. Food chemistry, 178: 106-114.
Wani, K. M., & Uppaluri, R. V. (2023). Characterization of pectin extracted from pomelo peel using pulsed ultrasound assisted extraction and acidic hot water extraction process. Applied Food Research, 3(2): 100345.
Woo, K. K., Chong, Y. Y., Li Hiong, S. K., & Tang, P. Y. (2010). Pectin extraction and characterization from red dragon fruit (Hylocereus polyrhizus): A preliminary study. Journal of Biological Sciences, 10(7): 631-636.
Ximenes, E., Kim, Y., Mosier, N., Dien, B., & Ladisch, M. (2011). Deactivation of cellulases by phenols. Enzyme and microbial technology, 48(1): 54-60.
Xu, Y., Zhang, L., Bailina, Y., Ge, Z., Ding, T., Ye, X., & Liu, D. (2014). Effects of ultrasound and/or heating on the extraction of pectin from grapefruit peel. Journal of Food Engineering, 126: 72-81.
Yang, Y., Wang, Z., Hu, D., Xiao, K., & Wu, J. Y. (2018). Efficient extraction of pectin from sisal waste by combined enzymatic and ultrasonic process. Food Hydrocolloids, 79: 189-196.
Yuliarti, O., Matia-Merino, L., Goh, K. T., Mawson, J., & Brennan, C. (2012). Characterisation of gold kiwifruit pectin isolated by enzymatic treatment. International Journal of Food Science and Technology, 47(3): 633-639.
Zoghi, A., Vedadi, S., Esfahani, Z. H., Gavlighi, H. A., & Khosravi-Darani, K. (2023). A review on pectin extraction methods using lignocellulosic wastes. Biomass Conversion and Biorefinery, 13(7): 5577-5589.