Research in Plant Metabolites

Research in Plant Metabolites

Determination of antioxidant activity, and antifungal effect of Ferula persica L hydroalcoholic extract on some fungal strains causing strawberry and grape fruits rot “in vitro”

Document Type : Original Article

Authors
Mostafa Rahmati-Joneidabad 1
Behrooz Alizadeh Behbahani 2
Mohammad Noshad 3
1 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
2 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.
3 Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan
10.22034/jrpsm.2024.165984
Abstract
In this study, the hydroalcoholic extract of Ferula persica was extracted based on soaking method, and then the content of total phenols, total flavonoids, free radical scavenging activity (based on DPPH and ABTS free radical scavenging methods) and antifungal effect (based on antimicrobial methods of disk diffusion agar, well diffusion agar, minimum inhibitory concentrations, and minimum fungicidal concentrations) were evaluated against fungi species causing strawberry and grape fruits rot (Botrytis cinerea, Aspergillus niger, and Rhizopus stolonifer). The results of this study showed that the hydroalcoholic extract of Ferula persica contained 101.38 mg GAE/g total phenols and 37.41 mg QE/g total flavonoids. In addition, the antioxidant activity of hydroalcoholic extract based on the percentage of inhibition of DPPH and ABTS free radicals was 68.59 and 63.32%, respectively. Findings of antifungal activity based on disk diffusion agar and well diffusion agar tests showed that the antifungal effect of the extract was dependent on its concentration and increasing the concentration caused a significant increase in the diameter of the growth inhibition zone. Rhizopus stolonifer and Aspergillus niger were the most resistant and sensitive fungal strains to the hydroalcoholic extract, respectively, and the minimum lethal concentrations for these two strains were 256 and 64 mg/ml, respectively. Therefore, Ferula persica hydroalcoholic extract can be used as a natural preservative to increase the shelf life of horticultural products.
Keywords
Ferula persica extract
Grape
Strawberry
Antifungal
Radical scavenging
Alizadeh Behbahani, B. A., & Fooladi, A. A. I. (2018). Evaluation of phytochemical analysis and antimicrobial activities Allium essential oil against the growth of some microbial pathogens. Microbial pathogenesis, 114, 299-303.
Alizadeh Behbahani, B. A., Noshad, M., & Falah, F. (2019). Study of chemical structure, antimicrobial, cytotoxic and mechanism of action of syzgium aromaticum essential oil on foodborne pathogens. Potravinarstvo, 13(1).
Alizadeh Behbahani, B., Falah, F., Vasiee, A., & Tabatabaee Yazdi, F. (2021). Control of microbial growth and lipid oxidation in beef using a Lepidium perfoliatum seed mucilage edible coating incorporated with chicory essential oil. Food science & nutrition, 9(5), 2458-2467.
Alizadeh Behbahani, B., Jooyandeh, H., Falah, F., & Vasiee, A. (2020). Gamma‐aminobutyric acid production by Lactobacillus brevis A3: Optimization of production, antioxidant potential, cell toxicity, and antimicrobial activity. Food Science & Nutrition, 8(10), 5330-5339.
Alizadeh-Behbahani, B., Tabatabaei-Yazdi, F., Shahidi, F., & Mohebbi, M. (2012). Antimicrobial activity of Avicennia marina extracts ethanol, methanol & glycerin against Penicillium digitatum (citrus green mold). Scientific Journal of Microbiology, 1(7), 147-151.
Banaeian, N., Omid, M., & Ahmadi, H. (2011). Energy and economic analysis of greenhouse strawberry production in Tehran province of Iran. Energy Conversion and management, 52(2), 1020-1025.
Barzegar, H., Alizadeh-Behbahani, B., & Mehrnia, M. A. (2020). Quality retention and shelf life extension of fresh beef using Lepidium sativum seed mucilage-based edible coating containing Heracleum lasiopetalum essential oil: an experimental and modeling study. Food Science and Biotechnology, 29(5), 717-728.
Ebrahimi Hemmati Kaykha, M., Jooyandeh, H., Alizadeh Behbahani, B., & Noshad, M. (2020). Antimicrobial potential of Cordia myxa fruit on pathogenic bacteria: A study “in vitro” laboratory conditions. Food Science and Technology 17(101), 71-80.
Jadidi, M., Vafaei, A. A., Miladi Gorgi, H., Babaei, S., & Abady, Akram. (2010). The effect of Ferula Persica L Extracts, (Sakbinag) on symptoms of morphine withdrawal and sleeping time in mice. Pajouhesh dar pezeshki, 34(4), 225-230. (In Persian)
Majidaee, E., Hosseyni Talei, S. R., Gholamnezhad, S., & Ebrahimzadeh, M. A. (2020). Comparing the Effect of Different Extraction Methods and the Role of Solvent Polarity on Total Phenolic and Flavonoid Contents and Antioxidant Activities of Ferula persica. Journal of Mazandaran University of Medical Sciences, 30(188), 26-39.
Mehdinia Lichaei, B., Esmaeilzadeh Kenari, R., & Dinpanah, Gh. (2018). Extraction of phenolic compounds and tocopherols from Ferula Persica and evaluating the effect of the extract on the stability of sunflower seed oil as an alternative to the synthetic antioxidant. Journal of Food Technology and Nutrition. 15(4), 81-90. (In Persian)
Mirjani, R., Shahverdi, A.-R., Iranshahi, M., Amin, G., & Shafiee, A. (2005). Identification of Antifungal Compounds from Ferula persica. var. persica. Pharmaceutical biology, 43(4), 293-295.
Mohammadi, A., Hashemi, M., & Hosseini, S. M. (2015). The control of Botrytis fruit rot in strawberry using combined treatments of Chitosan with Zataria multiflora or Cinnamomum zeylanicum essential oil. Journal of Food Science and Technology, 52(11), 7441-7448.
Namazi, P., Barzegar, H., Alizadeh Behbahani, B., & Mehrnia, M. A. (2021). Evaluation of functional groups of bioactive compounds, antioxidant potential, total phenolic and total flavonoid content of red bell pepper extracts. Iranian Journal of Food and Technology, 18 (113), 301-311.
Rahmati Joneidabad, M., & Alizadeh Behbahani, B. (2021). Boswellia Sacra essential oil: Antioxidant activity and antifungal effect on some spoilage fungi causing strawberry rot. Iranian Journal of Food and Technology, 18(114), 25-34. (In Persian)
Rahmati-Joneidabad, M., Alizade Behbahani, B., & Noshad, M. (2021). Antifungal effect of Satureja khuzestanica essential oil on Aspergillus niger, Botrytis cinerea, and Rhizopus stolonifer causing strawberry’s rot and mold. Food Science and Technology, 18(115), 171-180.
Rahmati-Joneidabad, M., Alizadeh Behbahani, B. (2021). Identification of chemical compounds, antioxidant potential, and antifungal activity of (Thymus daenensis) essential oil against spoilage fungi causing apple rot. Iranian Food Science and Technology Research Journal, 17(5), 691-700. (In Persian)
Salami, P., Ahmadi, H., & Keyhani, A. (2010a). Estimating the energy indices and profitability of strawberry production in Kamyaran zone of Iran. Energy, 1(1).
Salami, P., Ahmadi, H., Keyhani, A., & Sarsaifee, M. (2010b). Strawberry post-harvest energy losses in Iran. Researcher, 2(4), 67-73.
Shahverdi, A.-R., Iranshahi, M., Mirjani, R., Jamalifar, H., Amin, G., & Shafiee, A. (2005). Bioassay-guided isolation and identification of an antibacterial compound from Ferula persica var. persica roots. DARU Journal of Pharmaceutical Sciences, 13(1), 17-19.
Sosani Gharibvand, Z., Alizadeh Behbahani, B., Noshad, M., & Jooyandeh, H. (2020). Investigation of the functional groups of bioactive compounds, radical scavenging potential, antimicrobial activity and cytotoxic effect of Callistemon Citrinus aqueous extract on cell line HT29: A Laboratory Study. JRUMS, 19 (5), 463-484.
Taghinia, P., Haddad Khodaparast, M. H., & Ahmadi, M. (2019). Free and bound phenolic and flavonoid compounds of Ferula persica obtained by different extraction methods and their antioxidant effects on stabilization of soybean oil. Journal of Food Measurement and Characterization, 13(4), 2980-2987.
Tanavar, H., Barzegar, H., Alizadeh Behbahani, B., & Mehrnia, M. (2020). Mentha pulegium essential oil: chemical composition, total phenolic and its cytotoxicity on cell line HT29. Iranian Food Science and Technology Research Journal, 16(5), 643-653. (In Persian)
Yeganegi, M., Yazdi, F. T., Mortazavi, S. A., Asili, J., Alizadeh Behbahani, B., & Beigbabaei, A. (2018). Equisetum telmateia extracts: Chemical compositions, antioxidant activity and antimicrobial effect on the growth of some pathogenic strain causing poisoning and infection. Microbial pathogenesis, 116, 62-67.