Research in Plant Metabolites

Research in Plant Metabolites

The effect of mechanical and chemical treatments on germination characteristics of two species of hourglass (Passiflora. caerulea and Passiflora. edulis)

Document Type : Original Article

Authors
seyed najmmaddin mortazavi 1
razieh biglari farash 1
parinaz tallebi 2
1 Department of Horticultural Sciences - Faculty of Agriculture - Zanjan University - Zanjan - Iran
2 Department of Horticulture, Faculty of Agriculture, Zanjan University, Zanjan, Iran
10.22034/jrpsm.2024.473949.1042
Abstract
The seed germination of most species of Passiflora is unknown, and the long period of natural or induced dormancy causes irregular germination in them, which makes its commercial use difficult. The present research is aimed at investigating mechanical treatments including removal from the tip of the seed, removal from the bottom of the seed and chemical treatments including water at 35 degrees Celsius, gibberellic acid, normal vinegar, potassium nitrate, sulfuric acid, cow's milk and natural lemon juice in the germination of two species of amaryllis. It was done as a completely randomized design in three replications. The results showed that the difference between the treatments was significant. Potassium nitrate, gibberellic acid and cow's milk treatments caused an increase in P. edulis by 81.4, 79.5 and 77.2 percent respectively and in P. cearulea by 82.28, 63.33 and 81.66 percent respectively. Germination percentage. In the P.edulis species, potassium nitrate, gibberellic acid and cow's milk treatments were 75, 72.5 and 69.45%, respectively, and in the P.cearulea species, these treatments were 63.14, 12.66 and 8.61%, respectively, the speed Improve germination. This improvement was also observed in the traits of seed germ index, root length, stem length, leaf length and width, leaf area, fresh and dry weight of shoots and roots, and the amount of total antioxidants. Therefore, pre-germination treatments such as potassium nitrate, gibberellic acid and cow's milk can increase the germination potential and increase the use of this plant in breeding fields and for ornamental, medicinal and food purposes
Keywords
priming
seed dormancy
hourglass
potassium nitrate
Abdul-Baki, A.A., and Anderson, J. D. 1973. Vigor determination in soybean Seed by multiple, Criteria. Crop Science, 13: 630 - 633.
Adelani, D. O. 2009. Germination Potential and Seedling Vigour of African star apple (Chrysophyllum albidum G.Don).M.F Dissertation, Department of Forestry and Wildlife Management. Federal University of Agriculture, Abeokuta, 148 pp.
Adelani, D.O and Maisamari, I. J. 2016. Effect of fresh cow milk and coconut milk on the germination of baobab (Adansonia digitata) seeds. Biological and Environmental Sciences Journal for the Tropics, 8(4):7-16.
Ahmad, P., and Prasad, M. N. V. (Eds.). (2012). Environmental adaptations and stress tolerance of plants in the era of climate change. Springer Science & Business Media.
Alvandian, S., Vahedi, A., and Taghizade, R. 2013. The study of effect ultrasound and chilling on germination of Myrtus medicinal plant (Myrtus communis L.). Seed Research Journal, 3(3): 21-31. [In Persian with English Summary].
Baskin, J. M. and Baskin, C. C. 2004. A classification system for seed dormancy. Seed Science Research, 14: 1 - 16.
Bell, D. T., Rokich, D. P., McChesney, C. J. and Plummer, J. A., 1995. Effects of temperature, light and gibberellic acid on the germination of seeds of 43 species native to Western Australia. Journal of Vegetation Science, 6: 797-806.
Brosché, M., Schuler, M. A., and Kalbina, I. V. (2005). Gene expression and metabolite responses in Arabidopsis after mechanical wounding and insect feeding. Plant Physiology, 138(1), 790-805.
Butola, J. S. and Badola, H. K. 2004. Effect of pre-sowing treatment on seed germination and seedling vigour in Angelica glauca, a threatened medicinal herb. G.B. Pant Institute of Himalayan Environment and Development, Himachal Unit, Mohal- Kullu 175 126, India.
Campos, C. N., and Eiras, M. (2004). Organic fertilizers and tomato seed priming: Influence on plant growth and productivity. Brazilian Journal of Plant Nutrition, 30(1), 123-130.
Cárdenas, J., Carranza, C., Miranda, D. and Magnitskiy, S. 2013. Effect of GA3, KNO3, and removing of basal Point of seeds on germination of sweet granadilla (Passiflora ligularis Juss) and yellow Passion fruit (Passiflora edulis f. Flavicarpa). Revista Brasileira de Fruiticultura, 35(3): 853-859.
Chimichi, S., Mercati, V., Moneti, G., Raffaelli, A. and Toja, E. 1998. Isolation and characterization of an unknown flavonoid in dry extracts from P. incarnata. Nat Prod Lett, 11: 32 - 225.
Chisha-kasumu, E., Woodward, S. and Price, A. 2007. Comparison of the effect of mechanical scarification and gibberellic acid treatments on seed germination in Pterocarpus angolensis. Southern Hemisphere Forestry Journal, 69: 63 - 70.
Cirak, C., Ayan, A.K. and Kevseroglu, K. 2004. The effects of light and some presoaking on germination rate of St. John Worth (Hypericum perforatum L.) seeds. – Pakistan J. Biol. Sci., 7: 182- 186.
Copeland, L. O., 1995. Principles of seed science and technology. Springer. Norwell, USA, P. 411.
Delanoy, M., Van damme, P., Scheldeman, X. and Beltran, J. 2006. Germination of Passiflora mollissima (Kunth) L.H.Bailey, Passiflora tricuspis Mast. and Passiflora nov sp. Seeds. Scientia Horticulturae, Amsterdam, 110: 198 - 203.
Egli, D. B., Tekrony, D. M., and  Phillips, A. D. (1990). Seedbed conditions and seed priming effects on soybean seed germination and vigor. Agronomy Journal, 82(3), 559-563.
Ghasemi Ghasare, m. and Kafi, M. 2010. Scientific and practical floristry (first volume). Sixth edition. Razavi Publishing House, 310 pages.
Hamidi, A. and Naderi Arefi, A. 2013 Growth, dormancy and seed germination. First edition. Tehran University Publications. 452 pages.
Jokar, and V. 2012 Seeds of horticultural plants (fruit trees, vegetables, greenhouses, ornamental plants and their maintenance). First edition. Serva Publications, Voice of Christ. 630 pages.
Kavegar, M., Hosseini, M.V. and Rashedmorsal, M.H. 2013. Effect of different treatments on breaking dormancy and germination of seeds of Phalairs minor Retz. –J. Plant Prot. 27: 128 -131.
Khan, J., M. Rauf, Z. Ali, H. Rashid and Khattack, M.S. 1999. Different stratification techniques effects on seed germination of Pistachio. Pakistan Journal of Biological Sciences, 2: 1412 - 1414.
Leon, J., Rojo, E., and Sanchez-Serrano, J. J. (2001). Wound signaling in plants. Journal of Experimental Botany, 52(354), 1-9.
Marostega. T.N., Luz, P.B.D., Tavares, A.R., Neves, L.G. and Sobrinho, S.D.P. 2017. Methods of breaking seed dormancy for ornamental passion fruit species. Scientific Article, 23(1): 72 - 78.
Oliveiria, Jr M.X., Sao Jose A.R., Reboucas, T.N.H., Morais, O.M. and Dourado, F.W.N. 2010. Overcoming seed dormancy of passion fruit (Passiflora cincinnata Mast.). Revista Brasileira de Fruiticultura, 32: 584-590.
Orozco-Cardenas, M., and Ryan, C. A. (1999). Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway. Proceedings of the National Academy of Sciences, 96(11), 6553-6557.
Ożarowski, A. 1982. Ziołolecznictwo. Poradnik dla lekarzy. Wydawnictwo Lekarskie PZWL, Warszawa, 198 - 9.
Perez, J.O., Restepo, M., Jarvis, A., Solazar, M. and Caetano, C. 2007. Diversity of Colombian Passifloraceae biogeography and an updated list for conservation. Biota Colomb, 8(1): 1 - 45.
Ranal, M.A., and Santana, D.G. 2006. How and why to measure the germination process? Brazilian Journal of Botany, 29(1): 1 - 11.
Reginatto, F.H., Kauffmann, C., Shripsema, J., Guillaume, D., Gosmann, G. and Schenkel, E. 2001. Steroidal and triterpenoidal glucosides from Passilfora alata. J Braz Chem Soc, 12: 6 – 32.
Ruan, S., Xue, Q., and Tylkowska, K. (2002). The influence of priming on germination of rice seeds and seedling emergence and performance in flooded soils. Field Crops Research, 76(2), 75-85.
Saha, K., Lajis, N. H., Israf, D. A., Hamzah, A. S., Khozirah, S., Khamis, S., & Syahida, A. (2004). Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal plants. Journal of Ethnopharmacology, 92(2-3), 263-267.
Sharma, A.D., Rathore, S.V.S., Srinivasan, K. and Tyagi, R.Y. 2014. Comparison of various seed priming methods for seed germination, seedling vigour and fruit yield in okra (Abelmoschus esculentus L. Moench). Scientia Horticulturae, 165: 75-81.
Sharma, P., and Bhardwaj, R. (2007). Effects of 24-epibrassinolide and gibberellic acid on antioxidant enzymes in Brassica juncea L. Biologia Plantarum, 51(1), 183-186.
Shim, S.L., Cheol, M.J., Jang, Ch.S., Paul, R., and Wook, K. 2008. Effect of potassium Nitrate priming on seed Germination of seashore paspalum. Horticultural Science, PP: 2259-2262.
Stout, D. 1998. Rapid and synchronous germination of Cicer milkvetch seed following diurnal temperature priming. – Crop Sci. 181: 263-266.
Wang, J.H., Baskin, C.C., Chen, W., and Du, G.Z. 2010. Variation in seed germination between populations of five sub-alpine woody species from eastern Qinghai-Tibet Plateau following dry storage at low temperatures. Ecology Research, 25(1): 195 - 203.
Wiart, C. 2006. Medicinal plants classified in the family Passifloraceae. In: Medicinal plants of Asia and the Pacific. Taylor and Francis CRC, Boca Raton, London, New York. 101 - 106.