Research in Plant Metabolites

Research in Plant Metabolites

Effect of Sodium Nitroprusside on Morphophysiological Characteristics and Essential Oil Percentage of Basil at Different Hoagland Concentrations

Document Type : Original Article

Authors
Fatemeh Hashemi 1
SahebAli Bolandnazar 1
Saeideh Alizadeh Salteh 2
Alireza Motallebi 1
1 University of Tabriz
2 Department of horticultural scences, faculty of agriculture, university of Tabriz
10.22034/jrpsm.2025.512929.1059
Abstract
Considering the nutritional and medicinal value of basil, the aim of this study was to investigate the effect of different concentrations of triacantanol at two different Hoagland concentrations on the growth characteristics and essential oil percentage of this plant. In this experiment, to achieve the highest biomass to increase the active ingredient, plants were cultivated hydroponically and application of nutrient solution was performed with two concentrations of complete Hoagland and half-strength Hoagland, and the elicitor sodium nitroprusside was used in three concentrations (concentrations of 0, 13 and 26 mg/L), and the yield of vegetative and physiological traits and the percentage of essential oil were measured. The results of comparing the average data showed that sodium nitroprusside improved the physiological characteristics and essential oil percentage when using complete Hoagland in hydroponic cultivation, so that the highest chlorophyll index, flavonoid and essential oil percentage were obtained in the treatment of 26 mg/L sodium nitroprusside in complete Hoagland nutrient solution, and the lowest values of these traits were obtained in conditions without spraying and without using Hoagland nutrient solution. Therefore, with the aim of increasing the yield and also increasing the essential oil percentage, the use of a concentration of 26 mg/L sodium nitroprusside is recommended. Considering that the use of complete Hoagland nutrient solution in hydroponic cultivation conditions improved the physiological characteristics and increased the amount of essential oil of the plant, which can be economically and pharmacologically justified and can be used to increase secondary metabolites in basil plants.
Keywords
Growth stimulants
flavonoids
secondary metabolites
foliar spraying
Arab, S., Baradaran Firouzabadi, M., & Asgharipour, M. (2015). The effect of ascorbic acid and sodium nitro prusside sprayed on photosynthetic pigments and some traits of safflower in terms of deficit irrigation. Journal agriculture of Plant Production, 38(4), 14-25. (In Persian).
Arshan, K., Samsampour, D., & Pasalari, H. (2023). Effect of sodium nitroprusside (SNP) on morpho-physiological characteristics of peppermint (Mentha piperita L.) under salinity stress. Journal of Plant Production Research, 30(1), 85-102.  [In Persian] https://doi.org/10.22069/jopp.2022.20194.2931
Asadi Sanam, S., Zavareh, M., Pirdashti, H., & Hashempour, A. (2014). The effect of sodium nitroprusside (SNP) on some biochemical properties of barley seedlings in salinity. Journal of Plant Production Research, 21(3), 19-32. (In Persian).
Beligni, M.V., & Lamattina, L. (2000). Nitric oxide stimulates seed germination and de-etiolation, and inhibits hypocotyl elongation, three light inducible responses in plants. Planta, 210, 215–221. https://doi.org/10.1007/pl00008128
Bharti, P. K., Singh, S. K., & Kumari, S. (2023). Elicitors: Role in secondary metabolite production in medicinal plants. In: Genetic Manipulation of Secondary Metabolites in Medicinal Plant. Pp. 147-178. Springer Nature, Singapore.
Brito, C., Gonçalves, A., Silva, E., Martins, S., Pinto, L., Rocha, L., Arrobas, M., ˆAngelo Rodrigues, M., Moutinho-Pereira, J.M., & Correia, C. (2021). Kaolin foliar spray improves olive tree performance and yield under sustained deficit irrigation. Scientia Horticulturae, 277: 109795.
Chang, C.C., Yang, M.H., Wen, H.M., & Chern, J.C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of food and drug analysis, 10(3), 178-182. https://doi.org/10.38212/2224-6614.2748
Corpas, F. J., Leterrier, M., Valderrama, R., Airaki, M., Chaki, M., Palma, J. M., & Barroso, J. B. (2011). Nitric oxide imbalance provokes a nitrosative response in plants under abiotic stress. Plant Science, 181(5), 604-611.
Duan, X., Su, X., You, Y., Qu, H., Li, Y., & Jiang, Y. (2007). Effect of nitric oxide on pericarp browning of harvested longan fruit in relation to phenolic metabolism. Food Chemistry, 104(2), 571-576.
Duan, X., Su, X., You, Y., Qu, H., Li, Y. & Jiang, Y. (2007). Effect of nitric oxide on pericarp browning of harvested longan fruit in relation to phenolic metabolism. Food Chemistry, 104(2), 571-576.
El-Tayeb, M.A. (2005). Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Grows. Reg. 45: 3. 215-224.
Esmaeilzadeh Bahabadi, S., Rezaei, A., & Najafi, S.H. (2015). Nitric oxide Effect on growth and some physiological parameters of In vitro cultured lemon balm (Melissa officinalis L.). J. Cell. Tissue. 6: 195-203.
Fan, H., Guo, S., Jiao, Y., Zhang, R., & Li, J. (2007). Effects of exogenous nitric oxide on growth, active oxygen species metabolism, and photosynthetic characteristics in cucumber seedlings under NaCl stress. Front Agriculture China. 1:308–314.
Fan, H., Li, T., Guan, L., Li, Z., Cai, Y., & Lin, Y. (2012). Effect of exogenous nitric oxide on antioxidant and DNA methylation of Dendrobium huoshanense grown under drought stress. Plant Cell Tiss Organ Cult. 109: 307-314.
Fan, H.F., Du, C.X., Ding, L., & Xu, Y.L. (2014). Exogenous nitric oxide promotes waterlogging tolerance as related to the activities of antioxidant enzymes in cucumber seedlings. Russian Journal of Plant Physiology 61(3): 366-373.
Farouk, S., & Al-Huqail, A. A. (2020). Sodium nitroprusside application regulates antioxidant capacity, improves phytopharmaceutical production and essential oil yield of marjoram herb under drought. Industrial Crops and Products, 158, 113034.‏
Gohari, G., Alavi, Z., Esfandiari, E., Panahirad, S., Hajihoseinlou, S., & Fotopoulos, V. (2020). Interaction between hydrogen peroxide and sodium nitroprusside following chemical priming of Ocimum basilicum L. against salt stress. Physiologia plantarum, 168(2), 361-373.‏
Gonnella, M., F. Serio, G. Conversa., & Santamaria, P. (2003). Yield and Quality of Lettuce Grown in Floating System Using Different Sowing Density and Plant Spatial Arrangements, Acta Hort. 614:687-692.
Gregini, Sh., & Khorasaninejad, S. (2017). Effects of sodium nitroprusside on physiological, biochemical and essence characteristics of savory (Satureja khuzestanica) under deficit water regimes. Journal of Plant Production Research, 24(3), 55-70.
Grob, F., Durner, J., & Gaupels, F. (2013). Nitric oxide, antioxidants and prooxidants in plant defence responses. Plant Science. doi: 10.3389/fpls.
Hayat, S., M. Mori, J. Pichtel., & Ahmad, A. (2010). Nitric Oxide in Plant Physiology. Wiley-VCH -Weinheim. 210 p.
Heidari, M., & Minaei, A. (2014). The effect of drought stress and humic acid on
flower yield and concentration of high nutrients in borage (Borago officinalis L).
Journal of Plant Production Research, 21(1), 167-182. (In Persian with English abstract) https://dorl.net/dor/20.1001.1.23222050.1393.21.1.9.9
Jayasinghe, C., Gotoh, N., Aoki, T., & Wada, S. (2003). Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.). Journal of Agricultural and Food chemistry, 51(15): 4442-4449. ‏
Juliani, H.R., & Simon, J.E. (2002). Antioxidant activity of basil. Trends in new crops and new uses. ASHS Press, Alexandria, Vesicular-Mucorrizae, 9: 575-579.
Karplus P.A., Daniels M.J., & Herriot, J.R. (1991). Atomic structure of ferredoxin-NadpC reductase, prototype for a structurally novel flavoenzyme family. Science 251:60–66.
Kopyra, M., & Gwozdz, E. A. (2003). Nitric oxide stimulates seed germination and counteracts the inhibitory effect of heavy metals and salinity on root growth of Lupinus luteus. Plant Physiology and Biochemistry, 41: 1011–1017.
Laspina, N.V., Groppa, M.D., Tomaro, M.L., & Benavides, M.P. (2005). Nitric oxide protects sunflower leaves against Cd induced oxidative stress. Journal of Plant Sciences, 169, 323-330.
Li, Q.Y., Niu, H.B., Yin, J., Wang, M.B., Shao, H.B., Deng, D.Z., Chen, X.X., Ren, J.P., & Li, Y.C. (2008). Protective role of exogenous nitric oxide against oxidative stress induced by salt stress in barley (Hordeum vulgare). Colloids and Surfaces B: Biointerfa. 56: 220-225.
Loliger. J. (1991). The use of antioxidants in food. In Free Radicals and Food Additives; Aruoma, O. I, Halliwell, B, Eds; Taylor and Francis: London, pp: 129-150.
Maurya, A. K., & Rani, A. (2017) Nitric oxide (NO) and physio-biochemical adaptation in plants against stress. In:
Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends Plant Science. 7: 405-410.
Mohasseli, V., & Sadeghi, S. (2019). Exogenously applied sodium nitroprusside improves physiological attributes and essential oil yield of two drought susceptible and resistant specie of Thymus under reduced irrigation. Industrial Crops and Products, 130: 130-136.
Nasibi, F., & Kalantari, K.M. (2009). Influence of nitric oxide in protection of tomato seedling against oxidative stress induced by osmotic stress. Acta. Physiol. Planta. 31: 1037-1044.
Nasibi, F., & Kalantari, K. M. (2010). Influence of nitric oxide in protection of tomato seedling against oxidative stress induced by osmotic stress. Acta Physiology Plant, 31, 1037-1044.
Nasibi, P. (2011). The effect of different concentrations of sodium nitroprusside (SNP) at a discount oxidative damage induced by drought stress in tomato plants. J. Plant Biol. 2 (9): 74-63.
Palmgren, M. G., & Harper, J. F. (1999) Pumping with plant P-type ATPases. Journal of Experimental Botany, 50, 883-893.
Sandalio, L. M., Rodriguez-Serrano, M., Gupta, D. K., Archilla, A., Romero-Puertas, M. C., & del Rio, L. A. (2012) Reactive oxygen species and nitric oxide in plants under cadmium stress: from toxicity to signaling. In: Environmental Adaptations and Stress Tolerance of Plants in the Era of Climate Change (eds. Ahmad, P. and Prasad, M.) Springer, New York, NY.
Shehab, G.G., Ahmad, O.K., & EL- Beltagi, H.S. (2010). Effects of various chemical agents for alleviation of drought stress in rice plants. Notulae Botanicae Horti Agrobotanici, 38(1), 130-148.
Thakur, M., Bhattacharya, S., Khosla, P. K., & Puri, S. (2019). Improving production of plant secondary metabolites through biotic and abiotic elicitation. Journal of Applied Research on Medicinal and Aromatic Plants, 12, 1-12.
Tian, X., & Li, Y. (2006). Nitric oxide treatment alleviates drought stress in wheat seedlings. Biol. Planta. 50: 775-778.
Wu, F., Yang, H., Chang, Y., Cheng, j., Bai, sh., & Yin, j. (2012). Effects of nitric oxide on reactive oxygen species and antioxidant capacity in Chinese Bayberry during storage. Scientia Horticulturae. 135, 106–111.
Yasir, T.A., Khan, A., Skalicky, M., Wasaya, A., Rehmani, M.I.A., Sarwar, N., Mubeen, K., Aziz, M., Hassan, M.M., Hassan, F.A., & Iqbal, M.A. (2021). Exogenous sodium nitroprusside mitigates salt stress in lentil (Lens culinaris medik.) by affecting the growth, yield, and biochemical properties. Mole. 26: 9. 1-13.
Ye, L., Zhao, X., Bao, E., Cao, K., & Zou, Z. (2019). Effects of arbuscular mycorrhizal fungi on watermelon growth, elemental uptake, antioxidant, and photosystem II activities and stressresponse gene expressions under salinity-alkalinity stresses. Front. Plant Sci. 10: 2. 863-872.