Research in Plant Metabolites

Research in Plant Metabolites

The best time to measure the content of phenol , amino acids and photosynthetic pigments in response to foliar spraying of some chemical elicitors in lemongrass (Cymbopogon citratus)

Document Type : Original Article

Authors
Lida Barahmandzadeh 1
Alireza Abdali Mashhadi, 2
adel poshtdar 3
1 Department of Plant Production and Genetics, Faculty of Agriculture, Khuzestan University of Agricultural Sciences and Natural Resources, Ahvaz, Iran
2 Agronomy and Plant Breeding Department, Agriculture faculty, Khuzestan Agricultural Sciences and Natural Resources University, Ahvaz,. Iran
3 Department of Plant Production and Genetics, Faculty of Agriculture, Khuzestan University of Agriculture and Natural Resources, Ahvaz. Iran
10.22034/jrpsm.2025.507488.1052
Abstract
Lemon grass is one of the plants with essential oil of high economic value and many medicinal properties. This plant has phenolic and polyphenolic compounds, especially citral. To investigate the response of lemongrass to phenolic and amino acid chemical elicitors, a factorial experiment was conducted in the form of a completely randomized design with three replications in the research farm of the Agricultural Sciences and Natural Resources University of Khuzestan in 2021. The first factor includes different concentrations of chemical elicitors (salicylic acid in concentrations of 0, 50, 100, 200, and 400 μM and tryptophan amino acid in concentrations of 0, 50, 100, 200, and 400 mg/l) and the second factor includes the day of the sample. Harvesting was (2, 4, 6, and 8 days after spraying). The interaction effect of treatment was significant on all parameters except chlorophyll a, and six days after foliar spraying were the best time for sampling and measuring chlorophyll a. To measure total chlorophyll, chlorophyll b, and carotenoids in low concentrations of salicylic acid application, the best time was on the eighth day, but with high concentrations of salicylic acid and tryptophan amino acid, the fourth day was at the best time. The phenolic compounds of the whole plant were only affected by the concentration of 100 μM salicylic acid on the second day and 100 and 400 mg/L tryptophan on the eighth day. The highest amount of total phenol was observed at the most prolonged time interval after the application of tryptophan. The amino acid assay of the whole leaf also showed that the higher the concentration of the elicitors, the better the sampling, and the amino acid assay should be done in a longer time interval.
Keywords
biostimulants
Essential oil
Phenolic compounds
Salicylic acid
Tryptophan
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