Research in Plant Metabolites

Research in Plant Metabolites

Investigation of Physicochemical Properties and Bioactive Compounds of Fermented Ganoderma (Ganoderma lucidum)-Based Kombucha Compared to Green Tea and Coffee Kombucha

Document Type : Original Article

Authors
razieh biglari farash 1
Azizollah Khairy 2
1 Department of Horticultural Sciences, Zanjan University . Iran
2 Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
10.22034/jrpsm.2025.545805.1090
Abstract
Kombucha is a fermented tea beverage produced by the symbiosis of bacterial cultures and yeasts. Kombucha contains organic acids, minerals, and vitamins that mainly originate from the tea used in it. The type of tea has a significant effect on parameters related to antioxidant potential, pH, and also acetic acid, alcohol, or sugar content. Therefore, this experiment was designed and conducted as a factorial experiment in a completely randomized design in the tissue culture laboratory of the Horticulture Department of the University of Zanjan to compare three types of kombucha obtained from Ganoderma, green tea, and coffee. After preparing the three types of kombucha, the polyphenol, antioxidant, alcohol, sucrose, and acidity content indices were measured at time periods of 0, 5, 10, and 15 days. The results showed that fermentation time and tea type had a significant effect on the anti-radical property of kombucha at the 5% level. In terms of tea type, kombucha prepared from green tea had the highest antioxidant potential (94.61%) and phenolic content (320.1 mg/L) and flavonoids (254.1 mg/L), reaching its highest value on the first day of fermentation. Ganoderma ranked second and coffee ranked third. However, the stability of these compounds in Ganoderma was higher than others. The Ganoderma-containing beverage performed better in maintaining antioxidant capacity and increasing total phenols compared to coffee, although it was lower than that of green tea. However, due to the innovation and specific medicinal benefits of Ganoderma, it has great potential for the development of functional beverages.
Keywords
Antioxidant
Fermentation
Probiotic
Kombucha
Medicinal Mushrooms
Aspiyanto, A. S., Iskandar, J. M., Melanie, H., Maryati, Y., & Lotulung, P. D. (2017).
Characteristic of fermented spinach (Amaranthus spp.) polyphenol by kombucha culture for antioxidant
compound. AIP Conference Proceedings, 1803(1), 020018. https://doi.org/10.1063/1.4973140Ayed, L., & Hamdi, M. (2015). Manufacture of a beverage from cactus pear juice using “tea
fungus” fermentation. Annals of Microbiology, 65(4), 2293–2299. https://doi.org/10.1007/s13213-015-
1071
Ayed, L., Abid, S. B., & Hamdi, M. (2017). Development of a beverage from red grape juice
fermented with the kombucha consortium. Annals of Microbiology, 67(1), 111–121.
https://doi.org/10.1007/s13213-016-1242-2
Bell, V., Silva, C. R. P. G., Guina, J., & Fernandes, T. H. (2022). Mushrooms as future generation
healthy foods. Frontiers in Nutrition, 9, 1050099. https://doi.org/10.3389/fnut.2022.1050099
Bellassoued, K., Ghrab, F., Makni-Ayadi, F., Van Pelt, J., Elfeki, A., & Ammar, E. (2015).
Protective effect of Kombucha on rats fed a hypercholesterolemic diet is mediated by its antioxidant
activity. Pharmaceutical Biology, 53(11), 1699–1709.
https://doi.org/10.3109/13880209.2014.1001408
Četojević-Simin, D. D., Velićanski, A. S., Cvetković, D. D., Markov, S. L., Mrdanović, J. Ž.,
Bogdanović, V. V., & Šolajić, S. V. (2012). Bioactivity of lemon balm kombucha. Food and
Bioprocess Technology, 5(5), 1756–1765. https://doi.org/10.1007/s11947-011-0618-3
De Filippis, F., Troise, A. D., Vitaglione, P., & Ercolini, D. (2018). Different temperatures select
distinctive acetic acid bacteria species and promotes organic acids production during Kombucha tea
fermentation. Food Microbiology, 73, 275-283.
Ettayebi, K., Errachidi, F., Jamai, L., Tahri-Jouti, M. A., Sendide, K., & Ettayebi, M. (2003).
Biodegradation of polyphenols with immobilized Candida tropicalis under metabolic induction. FEMS
Microbiology Letters, 223(2), 215–219. https://doi.org/10.1016/S0378-1097(03)00380-4
Gaggìa, F., Baffoni, L., Galiano, M., Nielsen, D. S., Jakobsen, R. R., Castro-Mejía, J. L., Bosi,
S., Truzzi, F., Musumeci, F., & Dinelli, G. (2018). Kombucha beverage from green, black and rooibos
teas: A comparative study looking at microbiology, chemistry and antioxidant activity. Nutrients, 11(1),
1. https://doi.org/10.3390/nu11010001
Greenwalt, C. J., Steinkraus, K. H., & Ledford, R. A. (2000). Kombucha, the fermented tea:
Microbiology, composition, and claimed health effects. Journal of Food Protection, 63(7), 976–981.
https://doi.org/10.4315/0362-028X-63.7.976
Heidari, A., Rezazad Bari, M., & Amiri, S. (2018). Production of functional fermented Kombucha
drink using white tea and date syrup contains apple vinegar and ginger. Food Microbiology, 1, 27-38.
Hur, S. J., Lee, S. Y., Kim, Y.-C., Choi, I., & Kim, G.-B. (2014). Effect of fermentation on the
antioxidant activity in plant-based foods. Food Chemistry, 160, 346–356.
https://doi.org/10.1016/j.foodchem.2014.03.112
Jarrell, J., Cal, T., & Bennett, J. W. (2000). The Kombucha consortia of yeasts and bacteria.
Mycologist, 14(4), 166–170. https://doi.org/10.1016/S0269-915X(00)80034-8
Jayabalan, R., Malbaša, R. V., Lončar, E. S., Vitas, J. S., & Sathishkumar, M. (2014). A review
on kombucha tea—Microbiology, composition, fermentation, beneficial effects, toxicity, and tea
fungus. Comprehensive Reviews in Food Science and Food Safety, 13(4), 538–550.
https://doi.org/10.1111/1541-4337.12073
Kaashyap, M., Cohen, M., & Mantri, N. (2021). Microbial diversity and characteristics of
kombucha as revealed by metagenomic and physicochemical analysis. Nutrients, 13(12), 4446.
https://doi.org/10.3390/nu13124446
Kaewkod, T., Yingchaipreecha, K., & Aumprapha, W. (2019). Control of methanol during
fermentation of Kombucha for bacterial cellulose production. Foods, 8 (10), 451.
https://doi.org/10.3390/foods8100451
Karimi, R., Homayoonfal, M., Malekjani, N., Kharazmi, M. S., & Jafari, S. M. (2023).
Interaction between β-glucans and gut microbiota: a comprehensive review. Critical Reviews in Food
Science and Nutrition, 63 (25), 7804–7835. https://doi.org/10.1080/10408398.2023.2192281
Kim, J., & Adhikari, K. (2020). Current trends in kombucha: Marketing perspectives and the need
for improved sensory research. Beverages, 6(1), 15. https://doi.org/10.3390/beverages6010015
Kim, J., & Adhikari, K. (2020). Current trends in kombucha: Marketing perspectives and the
need for improved sensory research. Beverages, 6 (1), 15. https://doi.org/10.3390/beverages6010015
Marco, M. L., Sanders, M. E., Gänzle, M., Arrieta, M. C., Cotter, P. D., De Vuyst, L., Hill, C.,
Holzapfel, W., Lebeer, S., Merenstein, D., Reid, G., Wolfe, B. E., & Hutkins, R. (2021). The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on
fermented foods. Nature Reviews Gastroenterology & Hepatology, 18(3), 196–208.
https://doi.org/10.1038/s41575-020-00390-5
Ooi, T. S., Ting, A. S. Y., & Siow, L. F. (2020). Influence of selected native yeast starter cultures
on the antioxidant activities, fermentation index and total soluble solids of Malaysia cocoa beans: A
simulation study. LWT - Food Science and Technology, 122, 108977.
https://doi.org/10.1016/j.lwt.2019.108977
Paul, N. (2025). Significant role of bioactive compounds of Ganoderma lucidum in the aging
process. Open Access Research Journal of Biology and Pharmacy, 14(2), 035-044.
https://doi.org/10.53022/oarjbp.2025.14.2.0033
Pękal, A., & Pyrzynska, K. (2014). Evaluation of aluminium complexation reaction for flavonoid
content assay. Food Analytical Methods, 7(9), 1776–1782. https://doi.org/10.1007/s12161-014-9814
Pure, A. E., & Pure, M. E. (2016). Antioxidant and antibacterial activity of kombucha beverages
prepared using banana peel, common nettles and black tea infusions. Applied Food Biotechnology, 3(2),
125–130. https://doi.org/10.22037/afb.v3i2.11094
Saha, K., Lajis, N. H., Israf, D. A., Hamzah, A. S., Khozirah, S., Khamis, S., & Syahida, A.
(2004). Evaluation of antioxidant and nitric oxide inhibitory activities of selected Malaysian medicinal
plants. Journal of Ethnopharmacology, 92(2-3), 263-267
Saito, M. S., dos Santos, W. A., & Mamede, M. E. d. O. (2024). Coffee-Flavoured Kombucha:
Development, Physicochemical Characterisation, and Sensory Analysis. Fermentation, 10(7), 334.
https://doi.org/10.3390/fermentation10070334
Salafzoon, S., Mahmoodzadeh Hosseini, H., & Halabian, R. (2018). Evaluation of the antioxidant
impact of ginger-based kombucha on the murine breast cancer model. Journal of Complementary and
Integrative Medicine, 15(4), 1–8. https://doi.org/10.1515/jcim-2017-0071
Singleton V.L., Rossi J.A. Colorimetry of Total Phenolics with PhosphomolybdicPhosphotungstic Acid Reagents. Am. J. Enol. Vitic. 1965;16:144–158
Tanaka, T., Matsuo, Y., & Kouno, I. (2009). Chemistry of secondary polyphenols produced during
processing of tea and selected foods. International Journal of Molecular Sciences, 11(1), 14–40.
https://doi.org/10.3390/ijms11010014
Tu, C., Tang, S., Azi, F., Hu, W., & Dong, M. (2019). Use of kombucha consortium to transform
soy whey into a novel functional beverage. Journal of Functional Foods, 52, 81-89.
Vázquez-Cabral, B. D., Rocha-Guzmán, N. E., Gallegos-Infante, J. A., González-Herrera, S.
M., González-Laredo, R. F., Moreno-Jiménez, M. R., & Córdova-Moreno, I. T. S. (2014).
Chemical and sensory evaluation of a functional beverage obtained from infusions of oak leaves
(Quercus resinosa) inoculated with the kombucha consortium under different processing conditions.
Nutrafoods, 13(4), 169–178. https://doi.org/10.1007/s13749-014-0044
Velićanski, A. S., Cvetković, D. D., Markov, S. L., Tumbas Šaponjac, V. T., & Vulić, J. J.
(2014). Antioxidant and antibacterial activity of the beverage obtained by fermentation of sweetened
lemon balm (Melissa officinalis L.) tea with symbiotic consortium of bacteria and yeasts. Food
Technology and Biotechnology, 52(4), 420–429. https://hrcak.srce.hr/123664
Villarreal-Soto, S. A., Beaufort, S., Bouajila, J., Souchard, J. P., Renard, T., Rollan, S., &
Taillandier, P. (2020). Impact of fermentation conditions on the production of bioactive compounds
with anticancer, anti-inflammatory and antioxidant properties in kombucha tea extracts. Process
Biochemistry, 89, 110-120.
Vina, I., Semjonovs, P., Linde, R., & Deniņa, I. (2014). Current evidence on physiological activity
and expected health effects of kombucha fermented beverage. Journal of Medicinal Food, 17(2), 179–
188. https://doi.org/10.1089/jmf.2013.0031
Yavari, N., Assadi, M. M., Moghadam, M. B., & Larijani, K. (2011). Optimizing glucuronic acid
production using tea fungus on grape juice by response surface methodology. Australian Journal of
Basic and Applied Sciences, 5(9), 1788–1794.
Yavari, N., Mazaheri-Assadi, M., Mazhari, Z. H., Moghadam, M. B., & Larijani, K. (2017).
Glucuronic acid rich kombucha-fermented pomegranate juice. Journal of Food Research, 7(1), 61.
https://doi.org/10.5539/jfr.v7n1p61
Yavari, N., Mazaheri-Assadi, M., Moghadam, M. B., & Larijani, K. (2010). Response surface
methodology for optimization of glucuronic acid production using kombucha layer on sour cherry juice.
Australian Journal of Basic and Applied Sciences, 4(8), 3250–3256.
Zhao, Y. S., Eweys, A. S., Zhang, J. Y., Zhu, Y., Bai, J., Darwesh, O. M., Zhang, H. B., & Xiao,
X. (2021). Fermentation affects the antioxidant activity of plant-based food material through the release
and production of bioactive components. Antioxidants, 10 (12), 2004.
https://doi.org/10.3390/antiox10122004
Zhou, D. D., Li, J., Xiong, R. G., Saimaiti, A., Huang, S. Y., Wu, S. X., & Li, H. B. (2022).
Bioactive compounds, health benefits and food applications of kombucha tea: A comprehensive review.
Foods, 11(19), 2976.