Research in Plant Metabolites

Research in Plant Metabolites

The effect of different light intensities on biomass, protein and pigments of the cyanobacterium Spirulina platensis

Document Type : Original Article

Authors
1 گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران
2 Dept. of Plant Genetics and Production,,
Abstract
Spirulina (family Microcoleaceae) is a cyanobacterium formerly known as algae. Spirulina is highly valued in the animal feed, poultry and aquaculture industries, cosmetics and health care industries, as a source of energy and wastewater treatment. Spirulina is a rich source of protein, vitamins, minerals, fatty acids and pigments. These carotenoid pigments and phycobiliproteins (allophycocyanin, phycoerythrin and phycocyanin) have high commercial value. The pigment content depends on environmental conditions such as light intensity and light quality. In this study, the effect of light intensities (35, 55 and 75 μmol photons/m2s) on the biomass, protein, phycobiliprotein pigments and photosynthesis of Spirulina was investigated using a completely randomized design. The results showed that the best result was at a light intensity of 55 μmol photons/m2s in the production of phycobilin pigments, chlorophylls and pheophytin. While the highest protein and carotenoid pigments were produced at a light intensity of 75 μmol photons/m2s. Both light intensities of 55 and 75 μmol photons/m2s were suitable for biomass production, but the light intensity of 35 μmol photons/m2s was not sufficient for biomass and metabolite production in the cyanobacterium Spirulina. This study showed that different light intensities can have different effects on the biological factors of cyanobacteria.
Keywords

Aizpuru, A., & González-Sánchez, A. (2024). Traditional and new trend strategies to enhance
pigment contents in microalgae. World Journal of Microbiology and Biotechnology. 40(9): 1-26.
Anvar, A. A., & Nowruzi, B. )2021(. Bioactive properties of spirulina: A review. Microb.
Bioact. 4: 134-142.
Athiyappan, K. D., Routray, W., & Paramasivan, B. (2024). Phycocyanin from Spirulina: a
comprehensive review on cultivation, extraction, purification, and its application in food and allied
industries. Food and Humanity . 2: 100235.
Banayan, S., Jahadi, M., & Khosravi-Darani, K. (2022). Pigment productions by Spirulina
platensis as a renewable resource. Journal of Applied Biotechnology Reports. 9(2) : 614-621.
Banyan, S., Jihadi, M. & Fazel, M. (2020). Investigation of factors affecting the production of
chlorophyll and carotenoid pigments from Spirulina platensis using the Berman Platelet Design. Journal
of Food Microbiology. 2: 70-81. [In Persian]
Bennett, A., & Bogorad, L. (1973). Complementary chromatic adaptation in a filamentous bluegreen alga. The Journal of cell biology. 58(2): 419-435.
Bortolini, D. G., Maciel, G. M., Fernandes, I. D. A. A., Pedro, A. C., Rubio, F. T. V., Branco,
I. G., & Haminiuk, C. W. I. (2022). Functional properties of bioactive compounds from Spirulina spp.
Current status and future trends. Food Chemistry: Molecular Sciences, 5: 100134.
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities
of protein utilizing the principle of protein-dye binding. Analytical biochemistry. 72(1-2): 248-254.
Chilmawati, D., Martaningrum, A., Widowati, L. L., Candra, P., & Putra, P. (2024). Effect of
Different Purities of Seed Cells and Culture Media on the Growth Pattern and Protein Content of
Spirulina Platensis. Journal of Zoology and Systematics, 2(1): 49-58.
Dineshkumar, R., Narendran, R., & Sampathkumar, P. (2016). Cultivation of Spirulina
platensis in different selective media. NISCAIR-CSIR, India. 45(12): 1749-1754.
Habib, M. A. B., Parvin, M., Huntington, T. C., & Hasan, M. R. (2008). A review on culture,
production and use of Spirulina as food for humans and feeds for domestic animals. FAO Fisheries and
Aquaculture Circular No. 1034.
Hajong, S. Kumaria, S. & Tandon, P. (2019). Synergistic Effect of PPFD and Mycorrhization
for Efficient in vitro Propagation of Dendrobium chrysanthum Wall. ex Lindl. Int. J. Curr. Microbiol.
App. Sci. 8(10): 1290-1308.
Hotos, G. N. (2023). Quantity and Quality of Light on Growth and Pigment Content of Dunaliella
sp. and Anabaena sp. Cultures and the Use of Their Absorption Spectra as a Proxy Method for
Assessment. Journal of Marine Science and Engineering. 11(9): 1673.
Hynstova, V., Sterbova, D., Klejdus, B., Hedbavny, J., Huska, D., & Adam, V. (2018).
Separation, identification and quantification of carotenoids and chlorophylls in dietary supplements
containing Chlorella vulgaris and Spirulina platensis using high performance thin layer
chromatography. Journal of pharmaceutical and biomedical analysis. 148: 108-118.
Ismaiel, M. M., Piercey-Normore, M. D., & Rampitsch, C. (2018). Proteomic analyses of the
cyanobacterium Arthrospira (Spirulina) platensis under iron and salinity stress. Environmental and
Experimental Botany. 147: 63-74.
Jourdan, J. P. (2001). Grow your own Spirulina. Geneva Switz.
Jung, C. H., Waldeck, P., Sykora, S., Braune, S., Petrick, I., Küpper, J. H., & Jung, F. (2022).
Influence of different light-emitting diode colors on growth and phycobiliprotein generation of
Arthrospira platensis. Life. 12(6): 895.
Julianti, E., Susanti, S., Singgih, M., & Mulyani, L. N. (2019). Optimization of extraction method
and characterization of phycocyanin pigment from Spirulina platensis. J. Math. Fundam. Sci. 51: 168-
176.
Kumar, M., Kulshreshtha, J., & Singh, G. P. (2011). Growth and biopigment accumulation of
cyanobacterium Spirulina platensis at different light intensities and temperature. Brazilian Journal of
Microbiology. 42: 1128-1135.
Kusumaningtyas, P., Gultom, S. D., & Usman, U. (2023). Production of Photosynthetic Pigments
from Spirulina platensis Under Different Light Intensities. BERKALA SAINSTEK 11(3): 161-165.
Madhyastha, H. K., & Vatsala, T. M. (2007). Pigment production in Spirulina fussiformis in
different photophysical conditions. Biomolecular engineering. 24(3): 301-305.
Nunes, E., Odenthal, K., Nunes, N., Fernandes, T., Fernandes, I. A., & de Carvalho, M. A. P.
(2024). Protein extracts from microalgae and cyanobacteria biomass. Techno-functional properties and
bioactivity: A review. Algal Research. 82: 103638.
Nzayisenga, J. C., Farge, X., Groll, S. L., & Sellstedt, A. (2020). Effects of light intensity on
growth and lipid production in microalgae grown in wastewater. Biotechnology for Biofuels. 13: 1-8.
Palmer, J. S., Lawton, L. A., Kindt, R., & Edwards, C. (2021). Rapid analytical methods for the
microalgal and cyanobacterial biorefinery: Application on strains of industrial importance.
MicrobiologyOpen. 10(1): e1156.
Qiang, H., & Richmond, A. (1996). Productivity and photosynthetic efficiency of Spirulina
platensis as affected by light intensity, algal density and rate of mixing in a flat plate photobioreactor.
Journal of Applied Phycology. 8: 139-145.
Ravelonandro, P. H., Ratianarivo, D. H., Joannis‐Cassan, C., Isambert, A., &
Raherimandimby, M. (2008). Influence of light quality and intensity in the cultivation of Spirulina
platensis from Toliara (Madagascar) in a closed system. Journal of Chemical Technology and
Biotechnology: International Research in Process, Environmental and Clean Technology. 83(6): 842-
848.
Saini, D. K., Pabbi, S., & Shukla, P. (2018). Cyanobacterial pigments: Perspectives and
biotechnological approaches. Food and chemical toxicology. 120: 616-624.
Shah, M. A. R., Zhu, F., Cui, Y., Hu, X., Chen, H., Kayani, S. I., & Huo, S. (2024). Mechanistic
insights into the nutritional and therapeutic potential of Spirulina (Arthrospira) spp.: Challenges and
opportunities. Trends in Food Science and Technology. 104648.
Sinetova, M. A., Kupriyanova, E. V., & Los, D. A. (2024). Spirulina/Arthrospira/Limnospira—
Three Names of the Single Organism. Foods, 13(17): 2762.
Takano, H., Arai, T., Hirano, M., & Matsunaga, T. (1995). Effects of intensity and quality of
light on phycocyanin production by a marine cyanobacterium Synechococcus sp. NKBG 042902.
Applied Microbiology and Biotechnology. 43: 1014-1018.
Vasighi Jamil, F. & Nazeri, S. (2024). The effect of different NaCl concentrations and different
light spectra on the phycocyanin pigment of the cyanobacterium Spirulina. Thesis. Bu-Ali Sina
University, Hamadan. [In Persian]