مجله پژوهش در متابولیت‌های  گیاهی

مجله پژوهش در متابولیت‌های گیاهی

بررسی تاثیر نانوذرات دی‌اکسید تیتانیوم بر شاخص‌های بیوشیمیایی در مراحل مختلف تکوینی گل ناز یخی

نوع مقاله : مقاله پژوهشی

نویسندگان
گروه زیست شناسی گیاهی، دانشکده علوم طبیعی، دانشگاه تبریز، تبریز، ایران
چکیده
در سال‌های اخیر، نانوذرات دی‌اکسید تیتانیوم (TiO₂-NPs) به دلیل خواص فتوکاتالیستی و کاربردهای گسترده در علوم زیستی و کشاورزی، مورد توجه ویژه‌ای قرار گرفته‌اند. با این ‌حال، اثرات آن‌ها برفرآیندهای بیوشیمیایی گیاهان هنوز به‌طور کامل مشخص نشده است. مطالعه حاضر بر پایه یک طرح کاملا تصادفی با سه تکرار به بررسی مقایسه­ای تأثیر غلظت‌های مختلف TiO₂-NPs (0، 03/0 و 05/0 درصد) بر شاخص‌های بیوشیمیایی گیاه گل ناز یخیAptenia cordifolia) ) در دو مرحله رویشی و زایشی پرداخت و محتوای آنتوسیانین، فنل، فلاونوئید، آنزیم‌ آنتی‌اکسیدانی کاتالاز و قندهای محلول اندازه‌گیری شدند. نتایج نشان داد تیمار TiO₂-NPs اثر وابسته به غلظت و وابسته به مرحله رشدی دارد. به طوری که در مرحله رویشی و زایشی، غلظت 03/0 و 05/0 درصد موجب افزایش آنتوسیانین نسبت به گیاهان شاهد شد. میزان فنل در مرحله زایشی کاهش یافت که بیشترین کاهش در غلظت 03/0 درصد مشاهده شد اما فلاونوئید افزایش نشان داد که بیشترین افزایش در غلظت 05/0 درصد مشاهده شد و در مرحله رویشی این تغییرات معنادار نبودند. در هر دو مرحله رویشی و زایشی کاهش فعالیت آنزیم‌ کاتالاز مشاهده شد. در مرحله رویشی افزایش و در مرحله زایشی کاهش قندهای محلول مشاهده گردید. این نتایج نشان می‌دهد که استفاده از TiO₂-NPs اثرات متفاوتی بر فرآیندهای بیوشیمیایی گیاهان داشته و بیانگر پاسخ سازشی گیاه به تنش نانوذرات است.
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