مجله پژوهش در متابولیت‌های  گیاهی

مجله پژوهش در متابولیت‌های گیاهی

اثر محلول­پاشی اسید هیومیک و سیلیکون روی برخی خصوصیات کمی و کیفی بابونه (Matricaria chamomilla L.) در شرایط تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه علوم و مهندسی باغبانی دانشگاه بوعلی سینا ـ مجتمع آموزش عالی نهاوند (ویژه دختران)، همدان، ایران
2 دانشجوی کارشناسی رشته علوم و مهندسی باغبانی، دانشگاه بوعلی سینا ـ مجتمع آموزش عالی نهاوند (ویژه دختران)، همدان، ایران
10.22034/jrpsm.2026.545129.1089
چکیده
به منظور بررسی اثر محلول­پاشی اسید هیومیک و سیلیکون بر برخی خصوصیات کمی و کیفی بابونه تحت تنش شوری، آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی در سه تکرار در گلخانه تحقیقاتی مجتمع آموزش عالی نهاوند در سال 1403 اجرا شد. فاکتورهای مورد بررسی شامل تنش شوری در سه سطح (صفر، 50 و 100 میلی­مولار)، محلول­پاشی اسید هیومیک در سه سطح (صفر، 5/0 و 5/1 گرم در لیتر) و محلول­پاشی سیلیکون در سه سطح (صفر، 5/1 و 3 میلی­مولار) بود. صفاتی که در این آزمایش مورد اندازه­گیری قرار گرفتند، عبارت بودند از: محتوای کلروفیل a و b ، وزن تر و خشک گل، ارتفاع گیاه، قطر کاپیتول، محتوای فنل و فلاونوئید کل. نتایج، بیانگر تأثیر مثبت کاربرد اسید هیومیک و سیلیکون بر اکثر صفات مورد بررسی بود، به طوری که بیشترین میزان کلروفیل a (04/1 میلی­گرم بر گرم) و بیشترین میزان کلروفیل b (55/0 میلی­گرم بر گرم) در شوری 50 میلی­مولار و با استفاده از 5/0 گرم در لیتر اسید هیومیک و بدون کاربرد سیلیکون مشاهده شد. بیشترین مقادیر ارتفاع گیاه (41 سانتی­متر) و قطر کاپیتول (28/8 میلی­متر)، با کاربرد 50 میلی­مولار شوری و استفاده همزمان از 5/0 گرم در لیتر اسید هیومیک و 3 میلی­مولار سیلیکون به دست آمد. درصد افزایش این صفات در مقایسه با عدم کاربرد اسید هیومیک و سیلیکون در همان سطح شوری، به­ترتیب 39/24 و 55/23 درصد بود. بیشترین میزان فنل کل (07/13 میلی­گرم اسید گالیک بر گرم عصاره) و فلاونوئید کل (66/26 میلی­گرم روتین بر گرم عصاره)، در سطح شوری 100 میلی­مولار، 5/1 میلی­مولار سیلیکون و بدون استفاده از اسید هیومیک به دست آمد. با کاربرد همزمان اسید هیومیک و سیلیکون، آثار سوء تنش شوری در برخی صفات مانند ارتفاع گیاه و قطر کاپیتول کاهش یافت. بنابراین می­توان کاربرد اسید هیومیک و سیلیکون را به عنوان راهکاری مؤثر برای کاهش اثرات تخریبی تنش شوری پیشنهاد کرد.
کلیدواژه‌ها

Abdelaal, K.A.A., Mazrou, Y.S.A., & Hafez, Y.M. (2020). Silicon foliar application mitigates salt stress in sweet pepper plants by enhancing water status, photosynthesis, antioxidant enzyme activity and fruit yield. Plants, 9(6), Article 733, 1-15.
Aghaei Joubani, K., Taei, N., Kanani, M.R., & Yazdani, M. (2015). Effect of salt stress on some physiological and biochemical parameters of two Salvia species. Plant Process and Function, 3(9), 85-96. [In Persian]
Ahmad, P., Ahanger, M.A., Alam, P., Alyemeni, M.N., Wijaya, L., Ali, S., & Ashraf, M. (2019). Silicon (Si) supplementation alleviates NaCl toxicity in mung bean (Vigna radiata L.) through the modifications of physio-biochemical attributes and key antioxidant enzymes. Journal of Plant Growth Regulation, 38, 70-82.
Al-Amier, H., & Craker, L.E. (2007). In-vitro selection for stress tolerant spearmint. In: Janick, J. and Whipkey A. (eds), Issues in New Crops and New Uses, Pp. 306-310, ASHS Press.
Amiripour, A., Ghanbari Jahromi, M., Souri, M.K., & Mohammadi Torkashvand, A. (2021). Silicon stimulates physiochemical properties of coriander (Coriandrum sativum L.) to improve growth and yield under salt stress. Journal of Medicinal Plants and By-products, 2, 209-216.
Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiology, 24(1), 1-15.
Asemeh, M., & Pourakbar, L. (2016). Effect of silica nanoparticles on some growth factors in saffron plant under salinity stress. Iranian Biology Congress (19th National Congress and 7th International Congress), University of Tabriz and Iranian Biology Society, Tabriz. [In Persian]
Ashraf, M., & Harris, P.J.C. (2013). Photosynthesis under stressful environments: An overview. Photosynthetica, 51(2), 163-190.
Bacilio, M., Moreno, M., & Bashan, Y. (2016). Mitigation of negative effects of progressive soil salinity gradients by application of humic acids and inoculation with Pseudomonas stutzeri in a salt-tolerant and a salt-susceptible pepper. Applied Soil Ecology, 107, 394-404.
Bybordi, A. (2014). Interactive effects of silicon and potassium nitrate in improving salt tolerance of wheat. Journal of Integrative Agriculture, 13(9), 1889-1899.
Fatemi, H., Esmaiel Pour, B., Rizwan, M. (2020). Foliar application of silicon nanoparticles affected the growth, vitamin C, flavonoid, and antioxidant enzyme activities of coriander (Coriandrum sativum L.) plants grown in lead (Pb)-spiked soil. Environmental Science and Pollution Research, 28, 1417-1425.
Fauteux, F., Rémus-Borel, W., Menzies, J.G., & Bélanger, R.R. (2005). Silicon and plant disease resistance against pathogenic fungi. FEMS Microbiology Letters, Article 249, 1-6.
Gengmao, Z., Yu, H., Xing, S., Shihui, L., Quanmei, S., & Changhai, W. (2015). Salinity stress increases secondary metabolites and enzyme activity in safflower. Industrial Crops and Products, 64, 175-181.
Ghanavati, M., Houshmand, S., Zeinali, H., & Ebrahimpour, F. (2010). Chemical composition of the essential oils of Matricaria recutita L. belonging to central and south parts of Iran. Journal of Medicinal Plants, 9(34), 102-108. [In Persian]
Gülser, F., Sönmez, F., & Boysan Canal, S. (2010). Effects of calcium nitrate and humic acid on pepper seedling growth under saline condition. Journal of Environmental Biology, 31(5), 873-876.
Gutfinger, T. (1981). Polyphenols in olive oils. Journal of the American Oil Chemists Society, 58, 966-968.
Güzel, Y., Güzelşemme, M., & Miski, M. (2015). Ethnobotany of medicinal plants used in Antakya: A multicultural district in Hatay Province of Turkey. Journal of Ethnopharmacology, 174, 118-152.
Hajiboland, R., Cherghvareh, L., & Dashtebani, F. (2017). Effect of silicon supplementation on wheat plants under salt stress. Journal of Plant Process and Function, 5(18), 1-12.
Hasanuzzaman, M., Nahar, K., & Fujita, M. (2013). Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In: Ahmad, P., Azooz, M., Prasad, M. (eds), Ecophysiology and Responses of Plants under Salt Stress, Pp: 25-87, Springer, New York.
Hashemi, A., Abdolzadeh, A., & Sadeghipour, H.R. (2010). Beneficial effects of silicon nutrition in alleviating salinity stress in hydroponically grown canola, Brassica napus L. plants. Journal of Soil Science and Plant Nutrition, 56, 244-253.
Hurtado, A.C., Chiconato, D.A., Prado, R. de M., Sousa Junior, G. da S., Gratão, P.L., Felisberto, G., Viciedo, D.O., & Mathias dos Santos, D.M. (2020). Different methods of silicon application attenuate salt stress in sorghum and sunflower by modifying the antioxidative defense mechanism. Ecotoxicology and Environmental Safety, 203, Article 110964, 1-11.
Hussain, S.A., Farooq, M.A., Akhtar, J., & Saqib, Z.A. (2018). Silicon-mediated growth and yield improvement of sunflower (Helianthus annus L.) subjected to brackish water stress. Acta Physiologiae Plantarum, 40, 180.
Kim, Y-H., Khan, A.L., Waqas, M., & Lee, I-J. (2017). Silicon regulates antioxidant activities of crop plants under abiotic-induced oxidative stress: A review. Frontiers in Plant Science, 8, Article 510, 1-7.
Liang, Y., Zhang, W., Chen, Q., & Ding, R. (2005). Effects of silicon on H+-ATPase and H+-PPase activity, fatty acid composition and fluidity of tonoplast vesicles from roots of salt-stressed barley (Hordeum vulgare L.). Environmental and Experimental Botany, 53(1), 29-37.
Mali, M., & Aery, N.C. (2008). Influence of silicon on growth, relative water contents and uptake of silicon, calcium and potassium in wheat grown in nutrient solution. Journal of Plant Nutrition, 31(11), 1867-1876.
Meganid, A.S., Al-Zahrani, H.S., El-Metwally, Selim, M.S. (2015). Effect of humic acid application on growth and chlorophyll contents of common bean plants (Phaseolus vulgaris L.) under salinity stress conditions. International Journal of Innovative Research in Science, Engineering and Technology, 4(5), 2651-2660.
Menale, B., De Castro, O., Di Iorio, E., Ranaldi, M., & Muoio, R. (2021). Discovering the ethnobotanical traditions of the island of Procida (Campania, southern Italy). Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 156(2), 450-468.
Mikou, K., Rachiq, S., & Jarrar Oulidi, A. (2016). Étude ethnobotanique des plantes médicinales et aromatiques utilisées dans la ville de Fès au Maroc. Phytothérapie, 14, 35-43.
Ming, D.F., Pei, Z.F., Naeem, M.S., Gong, H.J., & Zhou, W.J. (2012). Silicon alleviates PEG‐induced water‐deficit stress in upland rice seedlings by enhancing osmotic adjustment. Journal of Agronomy and Crop Science, 198(1), 14-26.
Mohamed, W.H. (2012). Effects of humic acid and calcium forms on dry weight and nutrient uptake of maize plant under saline condition. Australian Journal of Basic and Applied Science, 6(8), 597-604.
Mohammed, A.E.M.E., Mewead, A.A.A., Gendy, A.S.H., & Abdelkader, M.A.I. (2019). Influence of humic acid rates and application times on vegetative growth and yield components of chamomile (Matricaria chamomilla L.) plants grown under reclaimed sandy soil conditions. Zagazig Journal of Agricultural Research, 46(6B), 2171-2181.
Mollazadeh, F., Ghanbari Jahromi, M., & Marashi, M. (2024). Alleviating salinity stress in Chinese lantern (Physalis alkekengi L.) by improving the plants growth characteristics and yield using zinc nanoparticle. Journal of Plant Process and Function, 14(65), 191-209. [In Persian]
Narimani, R., Moghaddam, M., Nemati, S.H., & Ghasemi Pirbaluti, A. (2018). Evaluation of salinity adjusted by using humic acid and ascorbic acid in medicinal plant of moldavian balm (Dracocephalum moldavica L.). Journal of Plant Research (Iranian Journal of Biology), 31(4), 927-938. [In Persian]
Neves, J.M., Matos, C., Moutinho, C., Queiroz, G., & Gomes, L.R. (2009). Ethnopharmacological notes about ancient uses of medicinal plants in Trás-os-Montes (northern of Portugal). Journal of Ethnopharmacology, 124(2), 270-283.
Ouni, Y., Ghnaya, T., Montemurro, F.. Abdelly, Ch., & Lakhdar, A. (2014). The role of humic substances in mitigating the harmful effects of soil salinity and improve plant productivity. International Journal of Plant Production, 8(3), 353-374.
Ozkan, G., Sagdic, O., Ekici. L., Ozturk, I., & Ozcan, M.M. (2007). Phenolic compounds of Origanum sipyleum L. extract, and its antioxidant and antibacterial activities. Journal of Food Lipids, 14(2), 157-169.
Petrakou, K., Iatrou, G., & Lamari, F.N. (2020). Ethnopharmacological survey of medicinal plants traded in herbal markets in the Peloponnisos, Greece. Journal of Herbal Medicine, 19, Article 100305.
Petronilho, S., Maraschin, M., Coimbra, M.A., & Rocha, S.M. (2012). In vitro and in vivo studies of natural products: A challenge for their valuation. The case study of chamomile (Matricaria recutita L.). Industrial Crops and Products, 40, 1-12.
Pizzeghello, D., Francioso, O., Ertani, A., Muscolo, A., & Nardi, S. (2013). Isopentenyladenosine and cytokinin-like activity of different humic substances. Journal of Geochemical Exploration, 129, 70-75.
Rady, M.M. (2012). A novel organo-mineral fertilizer can mitigate salinity stress effects for tomato production on reclaimed saline soil. South African Journal of Botany, 81, 8-14.
Raj, H., & Thakral, K.K. (2008). Effect of chemical fertilizers on growth, yield and quality of fennel (Foeniculum vulgare Miller). Journal of Spices and Aromatic Crops (JOSAC), 17(2), 134-139.
Rizwan, M., Ali, S., Ibrahim, M., Farid, M., Adrees, M., Bharwana, S.A., Zia-ur-Rehman, M., Qayyum, M.F., & Abbas, F. (2015). Mechanisms of silicon-mediated alleviation of drought and salt stress in plants: A review. Environmental Science and Pollution Research, 22(20), 15416-15431.
Robatjazi, R., Roshandel, P., & Hooshmand, S. (2020). Benefits of silicon nutrition on growth, physiological and phytochemical attributes of basil upon salinity stress. International Journal of Horticultural Science and Technology, 7(1), 37-50.
Rubio, V., Bustos, R., Irigoyen, M.L., Cardona-López, X., Rojas-Triana, M., & Paz-Ares, J. (2009). Plant hormones and nutrient signaling. Plant Molecular Biology, 69(4), 361-373.
Sakr, N. (2017). The Role of Silicon (Si) in increasing plant resistance against insect pests; Review article. Acta Phytopathologica et Entomologica Hungarica, 52(2), 185-204.
Samavat, S., & Malakooti, ​​M.J. (2006). The necessity of using organic acids (humic acid and fulvic acid) in increasing the quantity and quality of agricultural products. Technical bulletin No. 463, Soil and Water Research Institute, Agricultural Research and Education Organization, Ministry of Agricultural - Jahad. [In Persian]
Sarker, U., & Oba, S. (2020). The response of salinity stress-induced A. tricolor to growth, anatomy, physiology, non-enzymatic and enzymatic antioxidants. Frontiers in Plant Science, 11, Article 559876.
Silavi, B., & Eftekhari, S.A. (2016). Studying the effects of irrigation water salinity on leaf chlorophyll content and nutrient uptake of native Ahvaz lettuce by using different amounts of humic acid. 2nd National Conference on New Findings in Agricultural Sciences, Environment and Sustainable Natural Resources, Kerman, Jiroft. [In Persian]
Silva, O.N., Lobato, A.K.S., Ávila, F.W., Costa, R.C.L., Oliveira Neto, C.F., Santos Filho, B.G., Martins Filho, A.P., Lemos, R.P., Pinho, J.M., Medeiros, M.B.C.L., Cardoso, M.S., & Andrade, I.P. (2012). Silicon-induced increase in chlorophyll is modulated by the leaf water potential in two water-deficient tomato cultivars. Plant, Soil and Environment, 58(11), 481-486.
Tadayyon, M.R., Tadayyon, A., & Esmaili, S. (2021). Effect of humic acid on growth and yield indices of sunflower (Helianthus annuus) in a saline soil. Journal of Crop Production and Processing, 11(3), 101-110. [In Persian]
Tester, M., & Davenport, R. (2003). Na+ tolerance and Na+ transport in higher plants. Annals of Botany, 91(5), 503-527.
Torabi, F., Majd, A., & Enteshari, S. (2015). The effect of silicon on alleviation of salt stress in borage (Borago officinalis L.). Soil Science and Plant Nutrition, 61(5), 788-798.
Tripathi, D.K., Singh, S., Singh, V.P., Prasad, S.M., Dubey, N.K., & Chauhan, D.K. (2017). Silicon nanoparticles more effectively alleviated UV-B stress than silicon in wheat (Triticum aestivum) seedlings. Plant Physiology and Biochemistry, 110, 70-81.
Veisi, Z., Ghorbanpour, M., & Akramian, M. (2023). The effects of silicon nanoparticles on morpho-physiological and biochemical parameters of Calendula officinalis L. plants under salinity stress in hydroponic culture conditions. Plant Process and Function, 11(47), 211-229. [In Persian]
Wang, D., Hou, L., Zhang, L., & Liu, P. (2021). The mechanisms of silicon on maintaining water balance under water deficit stress. Physiologia Plantarum, 173(3), 1253-1262.
Yan, G-C., Miroslav, N., Ye, M-J., Xiao, Z-X., & Liang, Y-C. (2018). Silicon acquisition and accumulation in plant and its significance for agriculture. Journal of Integrative Agriculture, 17(10), 2138-2150.
Yin, L., Wang, S., Tanaka, K., Fujihara, S., Itai, A., Den, X., & Zhang, S. (2016). Silicon-mediated changes in polyamines participate in silicon-induced salt tolerance in Sorghum bicolor L. Plant, Cell and Environment, 39(2), 245-258.
Yoo, K.M., Lee, C.H., Lee, H., Moon, B., & Lee, C.Y. (2008). Relative antioxidant and cytoprotective activities of common herbs. Food Chemistry, 106, 929-936.
Zhu, Y-X., Gong, H-J., & Yin, J-L. (2019). Role of silicon in mediating salt tolerance in plants: A review. Plants, 8, Article 147, 1-22.
Živković, J., Ilić, M., Šavikin, K., Zdunić, G., Ilić, A., & Stojković, D. (2020). Traditional use of medicinal plants in south-eastern Serbia (Pčinja District): Ethnopharmacological investigation on the current status and comparison with half a century old data. Frontiers in Pharmacology, 11, Article 1020.